Topography of MECA-79-reactive L-selectin ligands in PLNs.
L1-2 cells that do not express L-selectin do not interact with venular endothelium, although some of these relatively large cells become mechanically retained in capillaries.
In the absence of antibody, L1-2 (L-selectin) transfectants roll in all venular orders, but not in arterioles.
After the recipient mouse was injected with 10 mg/kg MECA-79, L1-2 (L-selectin) cells fail to roll in high order venules, but several do roll in order I and II venules.
Animated 3-D reconstruction of serial confocal sections through a subiliac LNs 24 h after treatment with mAb MECA-79 and adoptive transfer of green fluorescent T cells.
M'Rini, C., Cheng, G., Schweitzer, C., Cavanagh, L.L., Palframan, R.T., Mempel, T., Warnock, R.A., Lowe, J.B., Quackenbush, E.J., and von Andrian, U.H. A novel endothelial L-selectin ligand activity in lymph node medulla that is regulated by alpha (1,3)-fucosyltransferase-IV. .J. Exp. Med. 198(9) : 1301-1312, 2003. [Abstract] [Full Text PDF] [Suppl. Fig. 1]
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