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Winner of the April 2003 BioRad Image Competition
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Section through a bone marrow cavity in the skull of a live mouse. The blood plasma is labelled with FITC-dextran (green). Note the rapid blood flow in the vessel center in the upper portion of the vessel. Rhodamine 6G (red) stains nuclei and mitochondria in the hematopoietic cells that adhere within the vessel (the round spheres insides the green detran-filled vessel) and the surrounding tissue. The cavity is enclosed in solid bone, which is not penetrated by rhodamine 6G and, hence, appears black. |
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Memory T cell homing
Localization of distinct antigen-experienced CD8+ T cell populations in secondary lymphoid tissues. The figure shows four three-color confocal micrographs of cryostat sections of spleens (upper row) and peripheral lymph nodes (lower row) from recipient mice 24 hours after intravenous injection of TRITC labeled cytotoxic effector cells (left column) or central memory cells (right column). The sections were counterstained with anti-B220 (green) and anti-Thy-1.2 (blue) to localize B cell follicles and T cell areas, respectively. TRITC labeled homed donor cells are identified by red fluorescence.
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| for more information on this topic see:
Weninger, W., Crowley, M.A., Manjunath, N. and von Andrian U.H. Migratory properties of naïve, effector, and memory CD8+ T cells. J. Exp. Med. 194(7): 953-966, 2001, 2001. [Abstract] [Full Text PDF] |
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False color photo showing platelet-mediated lymphocyte rolling via the interaction of P-selectin & Peripheral Node Addressin (PNAd)
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| for more information on this topic see:
Diacovo, T.G., Catalina, M.D., Siegelman, M.H., and von Andrian, U.H. Circulating activated platelets reconstitute lymphocyte homing and peripheral immunity in L-selectin deficient mice. J. Exp. Med. 187: 197-204, 1998. [Abstract] [Full Text PDF]
Diacovo, T.G., Puri, K.D., Springer, T.A., and von Andrian, U.H. Platelet-mediated lymphocyte delivery to high endothelial venules. Science 273: 252-255, 1996. [Abstract] [Full Text PDF] |
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False color photo of a mouse peripheral lymph node displaying Peripheral Node Addressin (PNAd) staining with CarboxyFluorescein Succinimidyl Ester (CFSE) conjugated Meca-79 antibody
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| for more information on this topic see:
M'Rini, C., Cheng, G., Schweitzer, C., Cavanagh, L.L., Palframan, R.T., Mempel, T., Warnock, R.A., Lowe, J.B., Quackenbush, E.J., and von Andrian, U.H. A novel endothelial L-selectin ligand activity in lymph node medulla that is regulated by alpha (1,3)-fucosyltransferase-IV. J. Exp. Med. 198(9): 1301-1312, 2003. [Abstract] [Full Text PDF]
Stockton, B.M., Ardman, B., Cheng, G., Manjunath, N., and von Andrian, U.H. Negative regulation of T cell homing by CD43. Immunity 8: 373-381, 1998. [Abstract] [Full Text PDF] |
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Three-step algorithm of lymphocyte homing to peripheral lymph nodes
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| for more information on this topic see:
Stein, J.V., Rot, A., Luo, Y., Narasimhaswamy, M., Nakano, H., Gunn, M.D., Matsuzawa, A., Quackenbush, E.J. Dorf, M.E., and von Andrian, U.H. The CC Chemokine Thymus-derived Chemotactic Agent 4 (TCA-4, Secondary Lymphoid Tissue Chemokine, 6Ckine, Exodus-2) Triggers Lymphocyte Function-associated Antigen 1-mediated Arrest of Rolling T Lymphocytes in Peripheral Lymph Node High Endothelial Venules. J. Exp. Med. 191: 61-75, 2000. [Abstract] [Full Text PDF]
Warnock, R.A., Askari, S., Butcher, E.C., and von Andrian, U.H. Molecular mechanisms of lymphocyte homing to peripheral lymph nodes. J. Exp. Med. 187: 205-216, 1998. [Abstract] [Full Text PDF] |
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