Lab Protocols

The protocols are contributed by members of the Springer Lab. More procotols will be added. If you would like to contribute protocols, please write them according to the Protocol Guidelines and e-mail the file in Word format and figures in GIF or JPEG format. The protocols were prepared to the best knowledge of the contributors. Springer Lab is not responsible for any unsatisfactory results. If you find a protocol helpful, please refer to this web site in your publications, and we will be glad to hear from you.

LAB COOKBOOK (available in downloadable PDF)

Protein Expression

• Establishment of Stable Transfectant of CHO Lec Cells by Jun Takagi
• Transient Transfection into 293T Cells by Jun Takagi
• Preparing a Selenomethionyl Protein by Sara Lindgren
• K562 Cell Transfection

Protein Purification and Characterization

• Purification of ICAM-1 by Mazen Ferzly
• Preparation of Tonsil Lysate by Mazen Ferzly
• [Sandwish] ELISA for Detecting Secreted ICAM-1 by Chang-Duk Jun
• On-column Protein Refolding

Radioactive Experiments

• Bench Top Radioactive Work Protocol by Mazen Ferzly
• Radioiodination by Jun Takagi

Immunochemical Methods

• Metabolic Labeling and Immunoprecipitation by Jun Takagi
• Semi-Quantitative Measurement of Proteins by Dot Blotting by Jun Takagi
• Purification of mAb (IgG) by Chang-Duk Jun
• Flow Cytometry Analysis by Qing Ma
• Animal Protocol by Qing Ma
• Fluorescently Labeling Antibodies

Cell Biology Protocols

• Chemotaxis Assay by Melissa Swope
• Purification of Human Mononuclear Cells and Neutrophils by Chang-Duk Jun
• Separation of Platelets from Whole Blood by Azucena Salas
• L-selectin-PNAd interactions under flow conditions. Flow Chamber Assay by Azucena Salas
• Cell Adhesion Assay